Serial Dilution Calculator

Get the simple step by step process to compute the serial dilution of a solution.

• Select the method i.e either dilution factor or concentration range to perform the serial dilution.
• Fill the details in the required input fields of the calculator.
• Tap the calculate button to check the repeat solutions parameters as output.

Serial dilution is a kind of solution dilution. A more exact definition is it is a stepwise dilution of a solution that is repeated a certain number of times and in which the concentration decreases with each and every step. Serial dilutions have many uses that are related to chemistry and biology.

The parameters of the serial dilution calculator are along the lines:

1. Method: We have two choices, one is the dilution factor and second is the concentration range. The concentration range requires to give the concentration of the initial and final solutions. The dilution factor takes the input as the dilution factor.
2. Number of dilutions: It is the number of solutions you are preparing. If you are making the starting solution from the stock solution, and alter the fields accordingly.
3. Number of uses per dilution: The number of repeats per solution.
4. Starting Solution Concentration: It is the concentration of the first solution in series.
5. Volume per use: How much solution you need per concentration of experiment
6. Error type: It can be either a percentage error or a pipette error.
7. Starting Solution Composition: It tells the starting volume needed and how to make that from the volume of the stock solution required, the volume of the dilutant be added.
8. Minimum volume required: The calculation is the volume per use * number of uses per dilution * error.
9. Repeat solution: It tells how to create each of the dilutions past the starting solution. It gives the volume to be transferred from one to the next dilution.
10. Dilution factor with respect to the starting solution: It takes the number of dilutions and the dilution factor and the final solution concentration if you're using the concentration range method
11. Concentration of solutions: It is the concentration of the first ten solutions.

• Determine the number of dilutions, dilution factor and starting solution concentration
• Find how much of the solution you require for each dilution.
• Calculate how much of the solution need to pipette from one dilution to the next.
• Make the starting solution.
• With a clean pipette, remove more volume from the starting solution into the next.
• Perform your experiment
• Dispose of all chemicals correctly and safely.

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1. What is a serial dilution?

Serial dilution is a series of sequential dilutions that are performed to convert a dense solution into a usable concentration.

2. How to calculate serial dilution using the calculator?

You have to select the method to perform serial dilution, enter a number of dilutions, dilution factor, starting solution concentration, volume to be left for each dilution, starting solution composition to check the repat solution details easily.

3. What are the applications of serial dilution?

Serial dilution is used in a number of experimental sciences like physics, pharmacology, biochemistry and homoeopathy.

• In biochemistry, serial dilution is used to find the desired concentration of reagents and chemicals from a higher concentration.
• In pharmacology, it is performed to receive the necessary concentration of chemicals and compounds.

4. What are the examples of serial dilution?

An example of serial dilution performed in our daily life is tea or coffee. In coffee, we add a certain amount of cold press coffee and add water so it obtains a desired concentration of coffee. Another simple example is the dilution of acids and bases in chemistry to obtain a required concentration.